Excitatory amino acid transporter 3 (SLC1A1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 430-524aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: AEDVTLIIAV DWLLDRFRTM VNVLGDAFGT GIVEKLSKKE LEQMDVSSEV NIVNPFALES TILDNEDSDT KKSYVNGGFA VDKSDTISFT QTSQF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Transports L-glutamate, L- and D-aspartate and L-cystein (PubMed:21123949). Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. Reference: "Loss-of-function mutations in the glutamate transporter SLC1A1 cause human dicarboxylic aminoaciduria."Bailey C.G., Ryan R.M., Thoeng A.D., Ng C., King K., Vanslambrouck J.M., Auray-Blais C., Vandenberg R.J., Broer S., Rasko J.E.J. Clin. Invest. 121:446-453(2011). Function: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate