EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 18-493aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal Flag-Myc-tagged. Target Protein Sequence: QYTEETITYT QCTDGYEWDP IRQQCKDIDE CDIVPDACKG GMKCVNHYGG YLCLPKTAQI IVNNEHPQQE TPAAEASSGA TTGTVAARSM ATSGVVPGGG FMASATAVAG PEVQTGRNNF VIRRNPADPQ RIPSNPSHRI QCAAGYEQSE HNVCQDIDEC TSGTHNCRTD QVCINLRGSF TCQCLPGYQK RGEQCVDIDE CTVPPYCHQR CVNTPGSFYC QCSPGFQLAA NNYTCVDINE CDASNQCAQQ CYNILGSFIC QCNQGYELSS DRLNCEDIDE CRTSSYLCQY QCVNEPGKFS CMCPQGYEVV RSRTCQDINE CETTNECRED EMCWNYHGGF RCYPRNPCQD HYVLTSENRC VCPVSNTMCR ELPQSIVYKY MSIRSDRSVP SDIFQIQATM IYANTINTFR IKSGNENGEF YLRQTSPVSA MLVLVKSLSG PREYIVDLEM LTVSSIGTFR TSSVLRLTII VGPFSF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Reference: "Lack of fibulin-3 causes early aging and herniation, but not macular degeneration in mice." McLaughlin P.J., Bakall B., Choi J., Liu Z., Sasaki T., Davis E.C., Marmorstein A.D., Marmorstein L.Y. Hum. Mol. Genet. 16:3059-3070(2007). Function: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth (By similarity).