Ectonucleoside triphosphate diphosphohydrolase 1 (Entpd1), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Baculovirus. Expression Region: 38-479aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: THNKPLPENV KYGIVLDAGS SHTNLYIYKW PAEKENDTGV VQLLEECQVK GPGISKYAQK TDEIAAYLAE CMKMSTERIP ASKQHQTPVY LGATAGMRLL RMESKQSADE VLAAVSRSLK SYPFDFQGAK IITGQEEGAY GWITINYLLG RFTQEQSWLN FISDSQKQAT FGALDLGGSS TQVTFVPLNQ TLEAPETSLQ FRLYGTDYTV YTHSFLCYGK DQALWQKLAQ DIQVSSGGIL KDPCFYPGYK KVVNVSELYG TPCTKRFEKK LPFNQFQVQG TGDYEQCHQS ILKFFNNSHC PYSQCAFNGV FLPPLQGSFG AFSAFYFVMD FFKKMANDSV SSQEKMTEIT KNFCSKPWEE VKASYPTVKE KYLSEYCFSG TYILSLLLQG YNFTGTSWDQ IHFMGKIKDS NAGWTLGYML NLTNMIPAEQ PLSPPLPHST YI. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Reference: "Kinetic characterization and immunodetection of ecto-ATP diphosphohydrolase (EC 3.6.1.5) in cultured hippocampal neurons." Boeck C.R., Sarkis J.J., Vendite D. Neurochem. Int. 40:449-453(2002). Function: nan