DNA repair protein RAD51 homolog 1 (RAD51), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-339aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AMQMQLEANA DTSVEEESFG PQPISRLEQC GINANDVKKL EEAGFHTVEA VAYAPKKELI NIKGISEAKA DKILAEAAKL VPMGFTTATE FHQRRSEIIQ ITTGSKELDK LLQGGIETGS ITEMFGEFRT GKTQICHTLA VTCQLPIDRG GGEGKAMYID TEGTFRPERL LAVAERYGLS GSDVLDNVAY ARAFNTDHQT QLLYQASAMM VESRYALLIV DSATALYRTD YSGRGELSAR QMHLARFLRM LLRLADEFGV AVVITNQVVA QVDGAAMFAA DPKKPIGGNI IAHASTTRLY LRKGRGETRI CKIYDSPCLP EAEAMFAINA DGVGDAKD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair. Reference: "Identification of a novel human Rad51 variant that promotes DNA strand exchange." Park J.Y., Yoo H.W., Kim B.R., Park R., Choi S.Y., Kim Y. Nucleic Acids Res. 36:3226-3234(2008). Function: nan