DNA polymerase theta (POLQ), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1820-2590aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-SUMO-tagged. Target Protein Sequence: SSSESLSIID VASDQNLFQT FIKEWRCKKR FSISLACEKI RSLTSSKTAT IGSRFKQASS PQEIPIRDDG FPIKGCDDTL VVGLAVCWGG RDAYYFSLQK EQKHSEISAS LVPPSLDPSL TLKDRMWYLQ SCLRKESDKE CSVVIYDFIQ SYKILLLSCG ISLEQSYEDP KVACWLLDPD SQEPTLHSIV TSFLPHELPL LEGMETSQGI QSLGLNAGSE HSGRYRASVE SILIFNSMNQ LNSLLQKENL QDVFRKVEMP SQYCLALLEL NGIGFSTAEC ESQKHIMQAK LDAIETQAYQ LAGHSFSFTS SDDIAEVLFL ELKLPPNREM KNQGSKKTLG STRRGIDNGR KLRLGRQFST SKDVLNKLKA LHPLPGLILE WRRITNAITK VVFPLQREKC LNPFLGMERI YPVSQSHTAT GRITFTEPNI QNVPRDFEIK MPTLVGESPP SQAVGKGLLP MGRGKYKKGF SVNPRCQAQM EERAADRGMP FSISMRHAFV PFPGGSILAA DYSQLELRIL AHLSHDRRLI QVLNTGADVF RSIAAEWKMI EPESVGDDLR QQAKQICYGI IYGMGAKSLG EQMGIKENDA ACYIDSFKSR YTGINQFMTE TVKNCKRDGF VQTILGRRRY LPGIKDNNPY RKAHAERQAI NTIVQGSAAD IVKIATVNIQ KQLETFHSTF KSHGHREGML QSDQTGLSRK RKLQGMFCPI RGGFFILQLH DELLYEVAEE DVVQVAQIVK NEMESAVKLS VKLKVKVKIG ASWGELKDFD V. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates high affinity thiamine uptake, probably via a proton anti-port mechanism. Has no folate transport activity. Reference: "Identification and characterization of the human and mouse SLC19A3 gene: a novel member of the reduced folate family of micronutrient transporter genes." Eudy J.D., Spiegelstein O., Barber R.C., Wlodarczyk B.J., Talbot J., Finnell R.H. Mol. Genet. Metab. 71:581-590(2000). Function: nan