Dipeptidase 3 (DPEP3), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 36-463aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: AETTPGAPRA LSTLGSPSLF TTPGVPSALT TPGLTTPGTP KTLDLRGRAQ ALMRSFPLVD GHNDLPQVLR QRYKNVLQDV NLRNFSHGQT SLDRLRDGLV GAQFWSASVS CQSQDQTAVR LALEQIDLIH RMCASYSELE LVTSAEGLNS SQKLACLIGV EGGHSLDSSL SVLRSFYVLG VRYLTLTFTC STPWAESSTK FRHHMYTNVS GLTSFGEKVV EELNRLGMMI DLSYASDTLI RRVLEVSQAP VIFSHSAARA VCDNLLNVPD DILQLLKKNG GIVMVTLSMG VLQCNLLANV STVADHFDHI RAVIGSEFIG IGGNYDGTGR FPQGLEDVST YPVLIEELLS RSWSEEELQG VLRGNLLRVF RQVEKVREES RAQSPVEAEF PYGQLSTSCH SHLVPQNGHQ ATHLEVTKQP TNRVPWRS. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human DPEP3 at 2 µg/mL can bind Anti-DPEP3 recombinant antibody (CSB-RA007125MA1HU).The EC50 is 6.841-7.498 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. Reference: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Gray A.M. Genome Res. 13:2265-2270 (2003) "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Sugano S. Nat. Genet. 36:40-45 (2004). Function: nan