Cytochrome P450 3A4 (CYP3A4), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 2-503aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: ALIPDLAMET WLLLAVSLVL LYLYGTHSHG LFKKLGIPGP TPLPFLGNIL SYHKGFCMFD MECHKKYGKV WGFYDGQQPV LAITDPDMIK TVLVKECYSV FTNRRPFGPV GFMKSAISIA EDEEWKRLRS LLSPTFTSGK LKEMVPIIAQ YGDVLVRNLR REAETGKPVT LKDVFGAYSM DVITSTSFGV NIDSLNNPQD PFVENTKKLL RFDFLDPFFL SITVFPFLIP ILEVLNICVF PREVTNFLRK SVKRMKESRL EDTQKHRVDF LQLMIDSQNS KETESHKALS DLELVAQSII FIFAGYETTS SVLSFIMYEL ATHPDVQQKL QEEIDAVLPN KAPPTYDTVL QMEYLDMVVN ETLRLFPIAM RLERVCKKDV EINGMFIPKG VVVMIPSYAL HRDPKYWTEP EKFLPERFSK KNKDNIDPYI YTPFGSGPRN CIGMRFALMN MKLALIRVLQ NFSFKPCKET QIPLKLSLGG LLQPEKPVVL KVESRDGTVS GA-. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds . Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position . Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone . Metabolizes testosterone to less biologically active 2beta- and 6beta-hydroxytestosterones . Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C-4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosynthesis . Catalyzes bisallylic hydroxylation of polyunsaturated fatty acids (PUFA) . Catalyzes the epoxidation of double bonds of PUFA with a preference for the last double bond . Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling . Plays a role in the metabolism of retinoids. Displays high catalytic activity for oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA) . Further metabolizes atRA toward 4-hydroxyretinoate and may play a role in hepatic atRA clearance . Responsible for oxidative metabolism of xenobiotics. Acts as a 2-exo-monooxygenase for plant lipid 1,8-cineole (eucalyptol) . Metabolizes the majority of the administered drugs. Catalyzes sulfoxidation of the anthelmintics albendazole and fenbendazole . Hydroxylates antimalarial drug quinine . Acts as a 1,4-cineole 2-exo-monooxygenase . Also involved in vitamin D catabolism and calcium homeostasis. Catalyzes the inactivation of the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) . Reference: "Effects of a commonly occurring genetic polymorphism of human CYP3A4 (I118V) on the metabolism of anandamide." Pratt-Hyatt M., Zhang H., Snider N.T., Hollenberg P.F. Drug Metab. Dispos. 38:2075-2082(2010). Function: nan