Cytochrome P450 3A13 (Cyp3a13), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-503aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MDLIPNFSME TWMLLATSLV LLYLYGTHSH GIFKKLGIPG PKPLPFLGTI LAYQKGFWEC DIQCHKKYGK MWGLYDGRQP VLAITDPDII KTVLVKECYS TFTNRRRFGP VGILKKAISI SENEEWKRIR ALLSPTFTSG RLKEMFPIIN QFTDVLVRNM RQGLGEGKPT SMKDIFGAYS MDVITATSFG VNIDSLNNPQ DPFVEKIKKL LKFDIFDPLF LSVTLFPFLT PVFDALNVSL FPRDVISFFT TSVERMKENR MKEKEKQRVD FLQLMINSQN YKTKESHKAL SDVEIVAQSV IFIFAGYETT SSALSFALYL LAIHPDVQKK LQDEIDAALP NKAPATYDTL LQMEYLDMVV NETLRLYPIA GRLERVCKTD VEINGLFIPK GTVVMIPTFA LHKDPKYWPE PEEFRPERFS KKNQDSINPY MYLPFGSGPR NCIGMRFALI NMKVALVRVL QNFTVQPCKE TEIPLKLSKQ GLLQPENPLL LKVVSRDETV SDE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Can activate aflatoxin B1 to a genotoxic product. Reference: "Molecular cloning and functional expression of a mouse cytochrome P-450 (Cyp3a-13): examination of Cyp3a-13 enzyme to activate aflatoxin B1 (AFB1)."Yanagimoto T., Itoh S., Sawada M., Hashimoto H., Kamataki T.Biochim. Biophys. Acta 1201:405-410(1994). Function: Can activate aflatoxin B1 to a genotoxic product.