Cytochrome P450 11B1, mitochondrial (CYP11B1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 25-503aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: GTRAARVPRT VLPFEAMPRR PGNRWLRLLQ IWREQGYEDL HLEVHQTFQE LGPIFRYDLG GAGMVCVMLP EDVEKLQQVD SLHPHRMSLE PWVAYRQHRG HKCGVFLLNG PEWRFNRLRL NPEVLSPNAV QRFLPMVDAV ARDFSQALKK KVLQNARGSL TLDVQPSIFH YTIEASNLAL FGERLGLVGH SPSSASLNFL HALEVMFKST VQLMFMPRSL SRWTSPKVWK EHFEAWDCIF QYGDNCIQKI YQELAFSRPQ QYTSIVAELL LNAELSPDAI KANSMELTAG SVDTTVFPLL MTLFELARNP NVQQALRQES LAAAASISEH PQKATTELPL LRAALKETLR LYPVGLFLER VASSDLVLQN YHIPAGTLVR VFLYSLGRNP ALFPRPERYN PQRWLDIRGS GRNFYHVPFG FGMRQCLGRR LAEAEMLLLL HHVLKHLQVE TLTQEDIKMV YSFILRPSMF PLLTFRAIN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes the hydroxylation of carbon hydrogen bond at 11-beta position of 11-deoxycortisol and 11-deoxycorticosterone/21-hydroxyprogesterone yielding cortisol or corticosterone, respectively. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Reference: n/a. Function: nan