Cystathionine beta-synthase (Cbs), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 2-561aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: PSGTSQCEDG SAGGFQHLDM HSEKRQLEKG PSGDKDRVWI RPDTPSRCTW QLGRAMADSP HYHTVLTKSP KILPDILRKI GNTPMVRINK ISKNAGLKCE LLAKCEFFNA GGSVKDRISL RMIEDAERAG NLKPGDTIIE PTSGNTGIGL ALAAAVKGYR CIIVMPEKMS MEKVDVLRAL GAEIVRTPTN ARFDSPESHV GVAWRLKNEI PNSHILDQYR NASNPLAHYD DTAEEILQQC DGKLDMLVAS AGTGGTITGI ARKLKEKCPG CKIIGVDPEG SILAEPEELN QTEQTAYEVE GIGYDFIPTV LDRAVVDKWF KSNDEDSFAF ARMLIAQEGL LCGGSSGSAM AVAVKAAREL QEGQRCVVIL PDSVRNYMSK FLSDKWMLQK GFMKEELSVK RPWWWRLRVQ ELSLSAPLTV LPTVTCEDTI AILREKGFDQ APVVNESGAI LGMVTLGNML SSLLAGKVRP SDEVCKVLYK QFKPIHLTDT LGTLSHILEM DHFALVVHEQ IQSRDQAWSG VVGGPTDCSN GMSSKQQMVF GVVTAIDLLN FVAAREQTQT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury. Reference: "The transcriptional landscape of the mammalian genome."Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005). Function: Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).