Cutinase (CUTA), Colletotrichum capsici, recombinant

Organism: Colletotrichum capsici (Anthracnose fungus). Source: E.coli. Expression Region: 17-228aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: APVEVGLDTG VANLEARQSS TRNELESGSS SNCPKVIYIF ARASTEPGNM GISAGPIVAD ALESRYGASQ VWVQGVGGPY SADLASNFII PEGTSRVAIN EAKRLFTLAN TKCPNSAVVA GGYSQGTAVM ASSISELSST IQNQIKGVVL SAITKNLQNL GRIPNFSTSK TEVYCALADA VCYGTLFILP AHFLYQADAA TSAPRFLAAR IG. Protein function: Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants (PubMed:33606796). Degrades cutin, a macromolecule that forms the structure of the plant cuticle (PubMed:33606796). Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of fungal infection. [The UniProt Consortium]. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection. Reference: "Structure of cutinase gene, cDNA, and the derived amino acid sequence from phytopathogenic fungi." Ettinger W.F., Thukral S.K., Kolattukudy P.E. Biochemistry 26:7883-7892(1987). Function: nan