Coagulation Factor X (human, recombinant)

Coagulation Factor X is a vitamin K-dependent plasma serine protease involved in the intrinsic, extrinsic, and common pathways of the blood coagulation cascade. It is composed of an N-terminal light chain that contains a signal sequence, a propeptide, carboxylated glutamic acid residues (Gla domain), and two EGF domains and a C-terminal heavy chain that contains the activation peptide and a chymotrypsin-like catalytic domain. Factor X is produced as an inactive precursor, which is activated to Factor Xa following signal peptide cleavage, glycosylation, and further cleavage by the Factor VIIIa-IXa or tissue factor-FVIIa complexes of the intrinsic or extrinsic pathways, respectively. Factor Xa, in complex with Factor Va, converts prothrombin to thrombin. Factor Xa also cleaves severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein, also known as surface glycoprotein, and prevents SARS-CoV-2 viral entry in vitro, and Factor X levels are increased in the lungs of patients with COVID-19. Knockout of F10, the gene encoding Factor X, in mice induces embryonic lethality and fatal internal bleeding of surviving neonates. Mutations in F10 result in Factor X deficiency, a rare bleeding disorder characterized by CNS and gastrointestinal bleeding, as well as hemarthroses and hematomas. Cayman's Coagulation Factor X (human, recombinant) protein consists of 468 amino acids, has a calculated molecular weight of 52.8 kDa, and a predicted N-terminus of Asn32 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular masses are 48 and 22 kDa, corresponding to the cleaved heavy and light chains, respectively, due to glycosylation.Synonyms: Factor 10, Factor X, FX, Stuart Factor, Stuart-Prower Factor, Thrombokinase. Purity: >97% estimated by SDS-PAGE. Endotoxin Testing: < 1.0 EU/µg, determined by the LAL endotoxin assay. Source: Recombinant human C-terminal His-tagged coagulation factor X expressed in insect cells. Amino Acids: 1-488 (full length). MW: 52.8 kDa. Formulation: (Request formulation change), Lyophilized from sterile 50 mM Tris, pH 8.0, 100 mM sodium chloride, and 10% glycerol. Host: Insect cells. UniProt Accession #: P00742.