Citrullinated Histone H4 (human, recombinant, His-tagged)

Histone H4 is one of four core histone proteins that is involved in the organization of DNA into chromatin. Histones are subject to a variety of post-translational modifications (PTMs), such as methylation, acetylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Histone H4 can be citrullinated at the arginine residue at position 3 (H4R3) by peptidyl arginine deiminase 4 (PAD4, Cay-10500). Citrullination of H4R3 increases in U2OS cells following induction of DNA damage by adriamycin (doxorubicin, Cay-15007) and is localized near fragmented nuclei. H4R3 citrullination is associated with smaller tumor size and inversely associated with p53 levels in tumor tissue samples derived from patients with non-small cell lung cancer (NSCLC). Citrullinated histone H4 is present in neutrophil extracellular traps (NETs) generated by stimulation of granulocytes with phorbol 12-myristate 13-acetate (PMA, Cay-10008014) and can be recognized by autoantibodies present in the serum of patients with rheumatoid arthritis. This product contains purified histone H4 (human, recombinant, His-tagged) (Cay-38257) that has been modified with PAD4, which is subsequently depleted by affinity chromatography.Purity: >80% estimated by SDS-PAGE. Source: Recombinant human N-terminal His-tagged histone H4 expressed in E. coli, citrullinated with recombinant human PAD4. Amino Acids: 1-103 (full length). MW: 13.4 kDa. Formulation: (Request formulation change), A solution in water. UniProt Accession #: P62805.