Citrullinated Histone H2B (human, recombinant)

Citrullinated Histone H2B Type 1-A is a citrullinated form of human histone H2B type 1-A (hTSH2B), a testis- and sperm-specific variant of the histone H2B protein that is encoded by H2BC1, previously known as HIST1H2BA. hTSH2B shares 85% identity with human somatic H2B.1, with the majority of amino acid differences between hTSH2B and somatic H2B occurring at the N-terminal end of the protein. H2BC1 is expressed in testis, and protein levels of hTSH2B are elevated in early round spermatids, decreasing over the course of spermiogenesis, with hTSH2B present in approximately 20% of mature spermatozoa. hTSH2B is enriched at promoter regions of genes important for sperm biology, capacitation, and fertilization and localizes to the basal nuclear area in mature spermatozoa. When incubated in Xenopus egg extracts, hTSH2B-positive human sperm cells decondense more rapidly and have increased long nuclear axis lengths compared with hTSH2B-negative sperm cells. Mice lacking the genes encoding the murine homolog of hTSH2B (TH2B), as well as the histone variant TH2A (Th2a-/-Th2b-/- mice), are sterile and have spermatogenesis defects. This product contains purified histone H2B type 1-A (human, recombinant) (Cay-11081) that has been modified with PAD4 enzyme, which is subsequently depleted by affinity chromatography. Cayman's Citrullinated Histone H2B Type 1-A (human, recombinant) can be used for Western blot and ELISA applications.Synonyms: H2BC1, HIST1H2BA, hTSH2B, TH2B, TSH2B.1. Purity: >90% estimated by SDS-PAGE. Protein Identity Confirmed by Mass Spec: Yes. Source: Recombinant human histone H2B type 1-A expressed in E. coli, citrullinated with human recombinant PAD4. Amino Acids: 2-127 (full length). MW: 14.04 kDa. Formulation: (Request formulation change), A solution in water. UniProt Accession #: Q96A08.