Chymotrypsin-C Protein (recombinant human)

Activity: Measured by its ability to cleave the fluorogenic peptide substrate, SUC­Ala­Ala­Pro­Phe­AMC.The specific activity is >300 pmol/min/µg. Protein Construction: Recombinant Human Chymotrypsin-C is produced by our Mammalian expression system and the target gene encoding Cys17-Leu268 is expressed with a 6His tag at the C-terminus. Sequence: Cys17-Leu268. Fusion tag: C-6His Endotoxin: < 1.0 EU per µg as determined by the LAL method. Apparent Molecular Mass: 35 kDa. Protein function: Chymotrypsin C (CTRC) is a member of the peptidase S1 family. CTRC is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. CTRC has broad substrate specificity, but prefers tocleave on the carboxyl side of hydrophobic residues. CTRC is expressed primarily in the pancreas, and is secreted into the digestive tract. CTRC plays a protective role in the pancreas by mitigating premature trypsinogen activation through degradation. It has been proposed that CTRC is a key regulator of digestive zymogen activation and is a physiological coactivator of digestive carboxypeptidases proCPA1 and proCPA2. The mutation of CTRC gene encodes the digestive enzyme CTRC contribute to the development of pancreatitis. Protein function: Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. [The UniProt Consortium]