Ceruloplasmin (CP), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 807-1050aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ILGPQLHADV GDKVKIIFKN MATRPYSIHA HGVQTESSTV TPTLPGETLT YVWKIPERSG AGTEDSACIP WAYYSTVDQV KDLYSGLIGP LIVCRRPYLK VFNPRRKLEF ALLFLVFDEN ESWYLDDNIK TYSDHPEKVN KDDEEFIESN KMHAINGRMF GNLQGLTMHV GDEVNWYLMG MGNEIDLHTV HFHGHSFQYK HRGVYSSDVF DIFPGTYQTL EMFPRTPGIW LLHCHVTDHI HAGM. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Ceruloplasmin is a blue, copper-binding glycoprotein. It has ferroxidase activity oxidizing Fe2+ to Fe3+ without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu2+ ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Reference: "Single-chain structure of human ceruloplasmin: the complete amino acid sequence of the whole molecule." Takahashi N., Ortel T.L., Putnam F.W. Proc. Natl. Acad. Sci. U.S.A. 81:390-394(1984). Function: nan