Catalase-3 (CAT3), Arabidopsis thaliana, recombinant

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: E.coli. Expression Region: 1-492aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MDPYKYRPSS AYNAPFYTTN GGAPVSNNIS SLTIGERGPV LLEDYHLIEK VANFTRERIP ERVVHARGIS AKGFFEVTHD ISNLTCADFL RAPGVQTPVI VRFSTVVHER ASPETMRDIR GFAVKFYTRE GNFDLVGNNT PVFFIRDGIQ FPDVVHALKP NPKTNIQEYW RILDYMSHLP ESLLTWCWMF DDVGIPQDYR HMEGFGVHTY TLIAKSGKVL FVKFHWKPTC GIKNLTDEEA KVVGGANHSH ATKDLHDAIA SGNYPEWKLF IQTMDPADED KFDFDPLDVT KIWPEDILPL QPVGRLVLNR TIDNFFNETE QLAFNPGLVV PGIYYSDDKL LQCRIFAYGD TQRHRLGPNY LQLPVNAPKC AHHNNHHEGF MNFMHRDEEI NYYPSKFDPV RCAEKVPTPT NSYTGIRTKC VIKKENNFKQ AGDRYRSWAP DRQDRFVKRW VEILSEPRLT HEIRGIWISY WSQADRSLGQ KLASRLNVRP SI. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Reference: "A chaperone function of NO CATALASE ACTIVITY1 is required to maintain catalase activity and for multiple stress responses in Arabidopsis." Li J., Liu J., Wang G., Cha J.Y., Li G., Chen S., Li Z., Guo J., Zhang C., Yang Y., Kim W.Y., Yun D.J., Schumaker K.S., Chen Z., Guo Y. Plant Cell 27:908-925(2015). Function: nan