Carbonic anhydrase 5B, mitochondrial (CA5B) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 34-317aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: CSLYTCTYKT RNRALHPLWE SVDLVPGGDR QSPINIRWRD SVYDPGLKPL TISYDPATCL HVWNNGYSFL VEFEDSTDKS VIKGGPLEHN YRLKQFHFHW GAIDAWGSEH TVDSKCFPAE LHLVHWNAVR FENFEDAALE ENGLAVIGVF LKLGKHHKEL QKLVDTLPSI KHKDALVEFG SFDPSCLMPT CPDYWTYSGS LTTPPLSESV TWIIKKQPVE VDHDQLEQFR TLLFTSEGEK EKRMVDNFRP LQPLMNRTVR SSFRHDYVLN VQAKPKPATS QATP. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: The esterase activity is determined to be 233 pmol/min/µg as measured under the described conditions. Form: Liquid. Buffer: 0.2 µm filtered 20 mM Tris-HCl, 100 mM NaCl, pH 8.0. Reconstitution: n/a. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide. CAs is associated with many biological processes, including calcification, respiration, bone resorption, acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart, pancreas, kidney, placenta, lung, and skeletal muscle, but it is restricted to the liver. CA5B is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA-VA. CA5B is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin, and Foscarnet. Reference: n/a. Function: Reversible hydration of carbon dioxide.