B-lymphocyte antigen CD20 (MS4A1), dog, recombinant

Organism: Canis familiaris (Dog) (Canis lupus familiaris). Source: in vitro E.coli expression system. Expression Region: 1-297aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MTTPRNSMSG TLPVDPMKSP TAMYPVQKII PKRMPSVVGP TQNFFMRESK TLGAVQIMNG LFHIALGSLL MIHTDVCAPI CITMWYPLWG GIMFIISGSL LAAADKNPRK SLVKGKMIMN SLSLFAAISG IIFLIMDIFN ITISHFFKME NLNLIKAPMP YVDIHNCDPA NPSEKNSLSI QYCGSIRSVF LGVFAVMLIF AFFQKLVTAG IVENEWKKLC SKPKSDVVVL LAAEEKKEQP IETTEEMVEL TEIASQPKKE EDIEIIPVQE EEGELEINFA EPPQEQESSP IENDSIP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. Reference: "Canine CD20 gene." Kano R., Inoue C., Okano H., Yamazaki J., Takahashi T., Watari T., Tokuriki M., Hasegawa A. Vet. Immunol. Immunopathol. 108:265-268(2005). Function: nan