Aurora B, Recombinant, Human (Serine/threnine Kinase) full length (AURKB)

Human Aurora kinase B (GenBank Accession No. NM_004217), (a.a. 2-344), expressed in an Baculovirus infected Sf9 cell expression system. Stability: 6 months at -80 °C , Purity: >95% by SDS-PAGE. Specific Activity: 22 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30 °C. , Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 0.2 ug Aurora B kinase., , Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.