Alpha-L-iduronidase (IDUA), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 28-653aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: APHLVHVDAA RALWPLRRFW RSTGFCPPLP HSQADQYVLS WDQQLNLAYV GAVPHRGIKQ VRTHWLLELV TTRGSTGRGL SYNFTHLDGY LDLLRENQLL PGFELMGSAS GHFTDFEDKQ QVFEWKDLVS SLARRYIGRY GLAHVSKWNF ETWNEPDHHD FDNVSMTMQG FLNYYDACSE GLRAASPALR LGGPGDSFHT PPRSPLSWGL LRHCHDGTNF FTGEAGVRLD YISLHRKGAR SSISILEQEK VVAQQIRQLF PKFADTPIYN DEADPLVGWS LPQPWRADVT YAAMVVKVIA QHQNLLLANT TSAFPYALLS NDNAFLSYHP HPFAQRTLTA RFQVNNTRPP HVQLLRKPVL TAMGLLALLD EEQLWAEVSQ AGTVLDSNHT VGVLASAHRP QGPADAWRAA VLIYASDDTR AHPNRSVAVT LRLRGVPPGP GLVYVTRYLD NGLCSPDGEW RRLGRPVFPT AEQFRRMRAA EDPVAAAPRP LPAGGRLTLR PALRLPSLLL VHVCARPEKP PGQVTRLRAL PLTQGQLVLV WSDEHVGSKC LWTYEIQFSQ DGKAYTPVSR KPSTFNLFVF SPDTGAVSGS YRVRALDYWA RPGPFSDPVP YLEVPVPRGP PSPGNP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: Generation and annotation of the DNA sequences of human chromosomes 2 and 4.Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.Nature 434:724-731(2005). Function: nan