Achaete-scute homolog 2 (Ascl2), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-263aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MEAHLDWYGV PGLQEASDAC PRESCSSALP EAREGANVHF PPHPVPREHF SCAAPELVAG AQGLNASLMD GGALPRLMPT SSGVAGACAA RRRQASPELL RCSRRRRSGA TEASSSSAAV ARRNERERNR VKLVNLGFQA LRQHVPHGGA NKKLSKVETL RSAVEYIRAL QRLLAEHDAV RAALAGGLLT PATPPSDECA QPSASPASAS LSCASTSPSP DRLGCSEPTS PRSAYSSEES SCEGELSPME QELLDFSSWL GGY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. Reference: "The human Achaete-Scute homologue 2 (ASCL2,HASH2) maps to chromosome 11p15.5, close to IGF2 and is expressed in extravillus trophoblasts." Alders M., Hodges M., Hadjantonakis A.-K., Postmus J., van Wijk I.J., Bliek J., de Meulemeester M., Westerveld A., Guillemot F., Oudejans C.B., Little P., Mannens M. Hum. Mol. Genet. 6:859-867(1997). Function: AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.