6-Phosphogluconate dehydrogenase, decarboxylating (PGD), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 4-483aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: ADIALIGLAV MGQNLILNMN DHGFVVCAFN RTVSKVDDFL ANEAKGTKVV GAQSLKEMVS KLKKPRRIIL LVKAGQAVDD FIEKLVPLLD TGDIIIDGGN SEYRDTTRRC RDLKAKGILF VGSGVSGGEE GARYGPSLMP GGNKEAWPHI KTIFQGIAAK VGTGEPCCDW VGDEGAGHFV KMVHNGIEYG DMQLICEAYH LMKDVLGMAQ DEMAQAFEDW NKTELDSFLI EITANILKFQ DTDGKHLLPK IRDSAGQKGT GKWTAISALE YGVPVTLIGE AVFARCLSSL KDERIQASKK LKGPQKFQFD GDKKSFLEDI RKALYASKII SYAQGFMLLR QAATEFGWTL NYGGIALMWR GGCIIRSVFL GKIKDAFDRN PELQNLLLDD FFKSAVENCQ DSWRRAVSTG VQAGIPMPCF TTALSFYDGY RHEMLPASLI QAQRDYFGAH TYELLAKPGQ FIHTNWTGHG GTVSSSSYNA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH. Reference: Identification of a cDNA encoding 6-phosphogluconate dehydrogenase from a human heart cDNA library.Tsui S.K.W., Chan J.Y., Waye M.M.Y., Fung K.-P., Lee C.-Y.Biochem. Genet. 34:367-373(1996). Function: Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.