1-Phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 (PLCD3), human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 1-789aa. Protein Length: Full Length. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: MLCGRWRRCR RPPEEPPVAA QVAAQVAAPV ALPSPPTPSD GGTKRPGLRA LKKMGLTEDE DVRAMLRGSR LRKIRSRTWH KERLYRLQED GLSVWFQRRI PRAPSQHIFF VQHIEAVREG HQSEGLRRFG GAFAPARCLT IAFKGRRKNL DLAAPTAEEA QRWVRGLTKL RARLDAMSQR ERLDHWIHSY LHRADSNQDS KMSFKEIKSL LRMVNVDMND MYAYLLFKEC DHSNNDRLEG AEIEEFLRRL LKRPELEEIF HQYSGEDRVL SAPELLEFLE DQGEEGATLA RAQQLIQTYE LNETAKQHEL MTLDGFMMYL LSPEGAALDN THTCVFQDMN QPLAHYFISS SHNTYLTDSQ IGGPSSTEAY VRAFAQGCRC VELDCWEGPG GEPVIYHGHT LTSKILFRDV VQAVRDHAFT LSPYPVILSL ENHCGLEQQA AMARHLCTIL GDMLVTQALD SPNPEELPSP EQLKGRVLVK GKKLPAARSE DGRALSDREE EEEDDEEEEE EVEAAAQRRL AKQISPELSA LAVYCHATRL RTLHPAPNAP QPCQVSSLSE RKAKKLIREA GNSFVRHNAR QLTRVYPLGL RMNSANYSPQ EMWNSGCQLV ALNFQTPGYE MDLNAGRFLV NGQCGYVLKP ACLRQPDSTF DPEYPGPPRT TLSIQVLTAQ QLPKLNAEKP HSIVDPLVRI EIHGVPADCA RQETDYVLNN GFNPRWGQTL QFQLRAPELA LVRFVVEDYD ATSPNDFVGQ FTLPLSSLKQ GYRHIHLLSK DGASLSPATL FIQIRIQRS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow. Regulates neurite outgrowth through the inhibition of RhoA/Rho kinase signaling. Reference: "Phospholipase C-delta3 binds with high specificity to phosphatidylinositol 4,5-bisphosphate and phosphatidic acid in bilayer membranes." Pawelczyk T., Matecki A. Eur. J. Biochem. 262:291-298(1999). Function: nan