Ac-Trp-Leu-Ala-AMC

Fluorogenic peptide substrate for measuring chymotrypsin-like activity of the 20S proteasome. This substrate is hydrolyzed by the beta5-subunit of the 20S proteasome to generate detectable fluorescence. 20S Proteasome enzyme requires activation with 0.035% SDS in the assay buffer. Excitation: 345nm. Emission: 445nm. Specific substrate to the constitutive proteasome, not hydrolyzed efficiently by the immunoproteasome. This substrate is useful for inhibitor screening and kinetic analysis.