Ac-DEVD-pNA

During apoptosis, activated caspase-3 cleaves several substrates, including poly(ADP-ribose) polymerase, which it specifically targets at the amino sequence DEVD. Ac-DEVD-pNA is a para-nitro aniline chromophore cleaved by caspases (Km = 18, 11, 32, 180, and 12 µM for caspases-1, -3, -4, -6, and -7, respectively). It is not cleaved by caspase-2. Cleavage is monitored colorimetrically at 405 nm.Formal Name: N-acetyl-L-alpha-aspartyl-L-alpha-glutamyl-L-valyl-N-(4-nitrophenyl)-L-alpha-asparagine. CAS Number: 189684-50-2. Synonyms: Ac-Asp-Glu-Val-Asp-pNA. Molecular Formula: C26H34N6O13. Formula Weight: 638.6. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: Acetonitrile:Methanol (1:1): 1 mg/ml. SMILES: CC(N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C(C)C)C(N[C@@H](CC(NC1=CC=C([N+]([O-])=O)C=C1)=O)C(O)=O)=O)=O)=O)=O. InChi Code: InChI=1S/C26H34N6O13/c1-12(2)22(25(41)30-18(26(42)43)10-19(34)28-14-4-6-15(7-5-14)32(44)45)31-23(39)16(8-9-20(35)36)29-24(40)17(11-21(37)38)27-13(3)33/h4-7,12,16-18,22H,8-11H2,1-3H3,(H,27,33)(H,28,34)(H,29,40)(H,30,41)(H,31,39)(H,35,36)(H,37,38)(H,42. InChi Key: DAOMBBSDYYRHPX-ORGXJRBJSA-N.