Ac-Ile-Glu-Thr-Asp-pNA

Description: Ac-Ile-Glu-Thr-Asp-pNA is a caspase-8 substrate that undergoes enzymatic binding and cleavage specifically at the Ile-Glu-Thr-Asp (IETD) peptide sequence, resulting in the release of p-nitroalinide, which can be quantitatively measured using colorimetric detection at 405 nm as an indicator of caspase-8 enzyme activity. Smiles: CC[C@H](C)[C@H](NC(C)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc(cc1)[N+]([O-])=O. References: Smith GK, et al. Expression, preparation, and high-throughput screening of caspase-8: discovery of redox-based and steroid diacid inhibition. Arch Biochem Biophys. 2002,399(2):195-205.