USP8 (Vector pENTR223.1, Accession No. BC110590)

Length: 3357 Sequence: ATGCCTGCTG TGGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT AAAAGACCTT AATAAGAAGA CAGAAGTTAA ACCAGAGAAA ATAAGCACTA AGAGTTATGT GCACAGTGCC CTGAAGATCT TTAAGACAGC AGAAGAATGC AGATTAGATC GTGATGAGGA AAGGGCCTAT GTACTATATA TGAAATACGT GACTGTTTAT AATCTTATCA AAAAAAGACC TGATTTCAAG CAACAGCAGG ATTATTTCCA TTCAATACTT GGACCTGGAA ACATCAAAAA AGCTGTCGAA GAAGCTGAAA GACTCTCTGA AAGCCTTAAA TTAAGATATG AAGAAGCTGA AGTCCGGAAA AAACTTGAGG AAAAAGACAG GCAGGAGGAA GCACAGCGGC TACAACAAAA AAGGCAGGAA ACAGGAAGAG AGGATGGTGG CACATTGGCT AAAGGCTCTT TGGAGAATGT TTTGGATTCC AAAGACAAAA CCCAAAAGAG CAATGGTGAA AAGAATGAAA AATGTGAGAC CAAAGAGAAA GGAGCAATCA CAGCAAAGGA ACTATACACA ATGATGACGG ATAAAAACAT CAGCTTGATT ATAATGGATG CTCGAAGAAT GCAGGATTAT CAGGATTCCT GTATTTTACA TTCTCTCAGT GTTCCTGAAG AAGCCATCAG TCCAGGAGTC ACTGCTAGTT GGATTGAAGC ACACCTGCCA GATGATTCTA AAGACACATG GAAGAAGAGG GGGAATGTGG AGTATGTGGT ACTTCTTGAC TGGTTTAGTT CTGCCAAAGA TTTACAGATT GGAACAACTC TCCGGAGTCT GAAAGATGCA CTTTTCAAGT GGGAAAGTAA AACTGTCCTG CGCAATGAGC CTTTGGTTTT AGAGGGAGGC TATGAAAACT GGCTCCTTTG TTATCCCCAG TATACAACAA ATGCTAAGGT CACTCCACCC CCACGACGCC AGAATGAAGA GGTGTCTATC TCATTGGATT TTACTTATCC CTCATTGGAA GAATCAATTC CTTCTAAACC TGCTGCCCAG ACGCCACCTG CATCTATAGA AGTAGATGAA AATATAGAAT TGATAAGTGG TCAAAATGAG AGAATGGGAC CACTGAATAT ATCAACTCCA GTTGAACCAG TTGCTGCTTC TAAATCTGAT GTTTCACCCA TAATTCAGCC AGTGCCTAGT ATAAAGAATG TTCCACAGAT TGATCGTACT AAAAAACCAG CAGTCAAATT GCCTGAAGAG CATAGAATAA AATCTGAAAG TACAAACCAT GAGCAACAAT CTCCTCAGAG TGGAAAAGTT ATTCCTGATC GTTCCACCAA GCCAGTAGTT TTTTCTCCAA CTCTCATGTT AACAGATGAA GAAAAGGCTC GTATTCATGC AGAAACTGCT CTTCTAATGG AAAAAAACAA ACAAGAAAAA GAACTTCGGG AAAGGCAGCA AGAGGAACAG AAAGAGAAAC TGAGGAAGGA AGAACAAGAA CAAAAAGCCA AAAAGAAACA AGAAGCTGAA GAAAATGAAA TTACAGAGAA GCAACAAAAA GCAAAAGAAG AAATGGAGAA GAAAGAAAGT GAACAGGCCA AGAAAGAAGA TAAAGAAACC TCAGCAAAGA GGGGCAAAGA AATAACAGGA GTAAAAAGAC AAAGTAAAAG TGAACATGAA ACTTCTGATG CCAAGAAATC TGTAGAAGAT AGGGGGAAAA GGTGTCCAAC CCCAGAAATA CAGAAAAAGT CAACAGGAGA TGTGCCCCAT ACATCTGTGA CAGGGGATTC AGGTTCAGGC AAGCCATTTA AGATTAAAGG ACAACCAGAA AGTGGAATTC TAAGGACAGG AACTTTTAGA GAGGATACAG ACGATACCGA AAGAAATAAA GCTCAACGAG AACCTTTGAC AAGAGCACGA AGTGAAGAAA TGGGGAGGAT CGTACCAGGA CTGCCTTCAG GCTGGGCCAA GTTTCTTGAC CCAATCACTG GAACCTTTCG TTATTATCAT TCACCCACCA ACACTGTTCA TATGTACCCA CCGGAAATGG CTCCTTCATC TGCACCTCCT TCCACCCCTC CAACTCATAA AGCCAAGCCA CAGATTCCTG CTGAGCGGGA TAGGGAACCT TCCAAACTGA AGCGCTCCTA CTCCTCCCCA GATATAACCC AGGCTATTCA AGAGGAAGAG AAGAGGAAGC CAACAGTAAC TCCAACAGTT AATCGGGAAA ACAAGCCAAC ATGTTATCCT AAAGCTGAGA TCTCAAGGCT TTCTGCTTCT CAGATTCGGA ACCTCAATCC TGTTTTTGGA GGTTCTGGAC CAGCTCTTAC TGGACTTCGT AACTTAGGAA ATACTTGTTA TATGAACTCA ATATTGCAGT GCCTATGTAA CGCTCCACAT TTGGCTGATT ATTTCAACCG AAACTGTTAT CAGGATGATA TTAACAGGTC AAATTTGTTG GGGCATAAAG GTGAAGTGGC AGAAGAATTT GGTATAATCA TGAAAGCCCT GTGGACAGGA CAGTATAGAT ATATCAGTCC AAAGGACTTT AAAATCACCA TTGGGAAGAT CAATGACCAG TTTGCAGGAT ACAGTCAGCA AGATTCACAA GAATTGCTTC TGTTCCTAAT GGATGGTCTC CATGAAGATC TAAATAAAGC TGATAATCGG AAGAGATATA AAGAAGAAAA TAATGATCAT CTCGATGACT TTAAAGCTGC AGAACATGCC TGGCAGAAAC ACAAGCAGCT CAATGAGTCT ATTATTGTTG CACTTTTTCA GGGTCAATTC AAATCTACAG TACAGTGCCT CACATGTCAC AAAAAGTCTA GGACATTTGA GGCCTTCATG TATTTGTCTC TACCACTAGC ATCCACAAGT AAATGTACAT TACAGGATTG CCTTAGATTA TTTTCCAAAG AAGAAAAACT CACAGATAAC AACAGATTTT ACTGCAGTCA TTGCAGAGCT CGACGGGATT CTCTAAAAAA GATAGAAATC TGGAAGTTAC CACCTGTGCT TTTAGTGCAT CTGAAACGTT TTTCCTACGA TGGCAGGTGG AAACAAAAAT TACAGACATC TGTGGACTTC CCGTTAGAAA ATCTTGACTT GTCACAGTAT GTTATTGGTC CAAAGAACAA TTTGAAGAAA TATAATTTGT TTTCTGTTTC AAATCACTAC GGTGGGCTGG ATGGAGGCCA CTACACAGCC TATTGTAAAA ATGCAGCAAG ACAACGGTGG TTTAAGTTTG ATGATCATGA AGTTTCTGAT ATCTCCGTTT CTTCTGTGAA ATCTTCAGCA GCTTATATCC TCTTTTATAC TTCATTGGGA CCACGAGTAA CTGATGTAGC Protein function: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation (PubMed:27302062). [The UniProt Consortium]