SHMT2 (Vector pENTR223.1, Accession No. BC011911)

Length: 1515 Sequence: atgctgtact tctctttgtt ttgggcggct cggcctctgc agagatgtgg gcagctggtc aggatggcca ttcgggctca gcacagcaac gcagcccaga ctcagactgg ggaagcaaac aggggctgga caggccagga gagcctgtcg gacagtgatc ctgagatgtg ggagttgctg cagagggaga aggacaggca gtgtcgtggc ctggagctca ttgcctcaga gaacttctgc agccgagctg cgctggaggc cctggggtcc tgtctgaaca acaagtactc ggagggttat cctggcaaga gatactatgg gggagcagag gtggtggatg aaattgagct gctgtgccag cgccgggcct tggaagcctt tgacctggat cctgcacagt ggggagtcaa tgtccagccc tactccgggt ccccagccaa cctggccgtc tacacagccc ttctgcaacc tcacgaccgg atcatggggc tggacctgcc cgatgggggc catctcaccc acggctacat gtctgacgtc aagcggatat cagccacgtc catcttcttc gagtctatgc cctataagct caaccccaaa actggcctca ttgactacaa ccagctggca ctgactgctc gacttttccg gccacggctc atcatagctg gcaccagcgc ctatgctcgc ctcattgact acgcccgcat gagagaggtg tgtgatgaag tcaaagcaca cctgctggca gacatggccc acatcagtgg cctggtggct gccaaggtga ttccctcgcc tttcaagcac gcggacatcg tcaccaccac tactcacaag actcttcgag gggccaggtc agggctcatc ttctaccgga aaggggtgaa ggctgtggac cccaagactg gccgggagat cccttacaca tttgaggacc gaatcaactt tgccgtgttc ccatccctgc aggggggccc ccacaatcat gccattgctg cagtagctgt ggccctaaag caggcctgca cccccatgtt ccgggagtac tccctgcagg ttctgaagaa tgctcgggcc atggcagatg ccctgctaga gcgaggctac tcactggtat caggtggtac tgacaaccac ctggtgctgg tggacctgcg gcccaagggc ctggatggag ctcgggctga gcgggtgcta gagcttgtat ccatcactgc caacaagaac acctgtcctg gagaccgaag tgccatcaca ccgggcggcc tgcggcttgg ggccccagcc ttaacttctc gacagttccg tgaggatgac ttccggagag ttgtggactt tatagatgaa ggggtcaaca ttggcttaga ggtgaagagc aagactgcca agctccagga tttcaaatcc ttcctgctta aggactcaga aacaagtcag cgtctggcca acctcaggca acgggtggag cagtttgcca gggccttccc catgcctggt tttgatgagc Protein function: Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:29364879, PubMed:25619277). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188). Also required for mitochondrial translation by producing 5,10- methylenetetrahydrofolate, 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640, PubMed:29364879). Associates with mitochondrial DNA (PubMed:18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985). [The UniProt Consortium]