RTF1 (Vector pUC, Accession No. BC015052)

Length: 1758 Sequence: atgaagaaac aggccaacaa aactgcctcc tcaggcagtt cagacaaaga cagttcagct gagagctcag cccctgagga aggtgaagtg tcagactctg acagcaacag ctcctcttcc agttcagatt cagactcttc ctcagaagat gaagagttcc atgatggcta tggagaagac ctcatgggag atgaggaaga cagggcccgt ctggaacaga tgacagagaa agagagagag caagaactgt tcaatcgcat agagaagagg gaggtgttga aaagaagatt tgaaatcaag aaaaaactaa aaacagccaa aaagaaagaa aagaaagaaa agaagaaaaa gcaagaagag gagcaagaaa agaaaaaact gacacagatt caagaatctc aggtaacatc ccacaacaag gaacggcgtt ccaagcggga tgagaaacta gacaagaaat ctcaagccat ggaggagcta aaagcagagc gagaaaaacg aaagaacaga acagctgagc tccttgccaa aaaacagcca ttaaaaacca gtgaggtcta ctctgatgat gaagaggagg aagaggatga caaatccagt gaaaagtcag accgctcatc acgaacatca tcgtctgatg aagaagagga gaaagaagag atccctccca aatcccaacc agtttcctta cctgaagaat tgaatcgggt tcgattatca cggcataagc tagaacgctg gtgtcacatg cccttctttg ctaaaactgt cacaggatgt tttgtgcgga ttggcatcgg aaaccacaac agcaaaccag tttaccgggt cgctgagatt acgggtgttg tggaaactgc caaagtttac caactaggtg gcaccagaac aaacaaaggg ctgcaactac ggcatggcaa tgaccaacgc gtgttccgtt tagagtttgt ctcaaaccaa gaattcaccg aaagtgagtt tatgaagtgg aaagaagcga tgttctctgc tggcatgcag ttgcccactc tagatgaaat caataaaaag gaattatcta ttaaagaagc tcttaattat aaattcaatg atcaggacat tgaagagatt gtaaaagaga aagaaaggtt cagaaaagct ccacccaact acgctatgaa gaagactcag ctactgaagg aaaaggccat ggctgaggac ctgggggatc aggacaaggc caaacaaatc caagatcaac tgaatgagct ggaggaacgg gcagaggccc tggaccgcca gcggaccaag aacatatccg ctatcagtta catcaaccag cggaaccggg agtggaacat tgtagagtct gagaaggccc ttgtggctga aagtcacaac atgaaaaacc aacagatgga tccctttact cggcggcagt gcaagcctac catcgtttct aattccagag acccagctgt tcaagctgcc atcttggccc agctgaatgc aaaatacggt tctggagtgt taccagatgc tccaaaggaa atgagcaagg gtcaaggcaa agataaagat ttgaattcta agtcagccag tgacctctca gaagatctgt tcaaagtaca cgattttgat gtgaagattg acttacaagt tcccagctca gagtcaaagg ctttagccat cacctccaag gctccgccag ccaaggatgg ggctccaagg agatctctga acttggaaga ctacaaaaaa cgacgagggc Protein function: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser- 5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency, this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex. [The UniProt Consortium]