COX5B (Vector pUC, Accession No. BC006229)

Length: 390 Sequence: atggcttcaa ggttacttcg cggagctgga acgctggccg cgcaggccct gagggctcgc ggccccagtg gcgcggccgc gatgcgctcc atggcatctg gaggtggtgt tcccactgat gaagagcagg cgactgggtt ggagagggag atcatgctgg ctgcaaagaa gggactggac ccatacaatg tactggcccc aaagggagct tcaggcacca gggaagaccc taatttagtc ccctccatct ccaacaagag aatagtaggc tgcatctgtg aagaggacaa taccagcgtc gtctggtttt ggctgcacaa aggcgaggcc cagcgatgcc cccgctgtgg agcccattac aagctggtgc cccagcagct Protein function: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [The UniProt Consortium]