Glycochenodeoxycholic Acid-d4

An internal standard for the quantification of glycochenodeoxycholic acid (GCDCA, Cay-16942) by GC- or LC-MS. GCDCA is a glycine-conjugated form of the primary bile acid chenodeoxycholic acid (Cay-10011286). It reduces formation of cholic acid (Cay-20250) in primary human hepatocytes when used at a concentration of 100 µM. GCDCA (50, 75, and 100 µM) reduces the number of LC3 puncta, a marker of autophagy, and is cytotoxic to L-02 hepatocytes. GCDCA (50 µM) induces apoptosis in isolated rat hepatocytes, an effect that can be blocked by the protein kinase C (PKC) inhibitor chelerythrine (Cay-11314). Fecal levels of GCDCA are decreased in a rat model of high-fat diet-induced obesity compared with rats fed a normal diet.Formal Name: N-[(3alpha,5beta,7alpha)-3,7-dihydroxy-24-oxocholan-24-yl-2,2,4,4-d4]-glycine. CAS Number: 1201918-16-2. Synonyms: GCDCA-d4. Molecular Formula: C26H39D4NO5. Formula Weight: 453.7. Purity: >99% deuterated forms (d1-d4). Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMSO: 10 mg/ml, DMSO:PBS(pH 7.2) (1:4): 0.2 mg/ml, Ethanol: 1 mg/ml. SMILES: C[C@H](CCC(NCC(O)=O)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@H](O)C[C@]4([H])C([2H])([2H])[C@H](O)C([2H])([2H])C[C@]4(C)[C@@]3([H])CC[C@@]21C. InChi Code: InChI=1S/C26H43NO5/c1-15(4-7-22(30)27-14-23(31)32)18-5-6-19-24-20(9-11-26(18,19)3)25(2)10-8-17(28)12-16(25)13-21(24)29/h15-21,24,28-29H,4-14H2,1-3H3,(H,27,30)(H,31,32)/t15-,16+,17-,18-,19+,20+,21-,24+,25+,26-/m1/s1/i8D2,12D2. InChi Key: GHCZAUBVMUEKKP-PKIQRPOYSA-N.