Protonex(TM) Lyso-Red 670

Molecular weight: 710.72. Excitation (nm): 643. Emission (nm): 660. Lysosomes are membrane-bound organelles containing acid hydrolases that break down biomolecules in an acidic environment (pH 4.5-4.8). They play critical roles in cellular processes by eliminating waste and debris, facilitating autophagy for organelle recycling, and mediating digestion through endocytosis and phagocytosis. Lysosomes aid cellular metabolism by converting complex molecules into usable forms and contribute to cell signaling, influencing growth, differentiation, and responses to signals. Dysfunctional lysosomes are associated with various diseases called lysosomal storage disorders, where the accumulation of undigested substances within lysosomes leads to cellular damage and systemic health issues. Notably, lysosomal dysfunction has been implicated in neurodegenerative diseases, cancer, and other disorders. Protonex(TM) Lyso-Red 670 is a cell-permeable, far-red fluorescent probe that selectively stains and visualizes acidic organelles within live cells, notably lysosomes. It remains quiescent or weakly fluorescent under alkaline pH conditions, exhibiting significant fluorescence enhancement as acidity increases. This property enables researchers to trace lysosomal dynamics (e.g., trafficking, fusion, fission, autophagy) in real-time via fluorescence microscopy techniques. Protonex(TM) Lyso-Red 670 has an excitation and emission maximum of 640 and 680 nm, respectively, and can be efficiently excited using a Cy5 filter.