mFluor(TM) Blue 630 Styramide

Absorbance (nm): 472. Correction Factor (260 nm): 0.197. Correction Factor (280 nm): 0.275. Extinction coefficient (cm^-1 M^-1): 49000 (PBS). Excitation (nm): 470. Emission (nm): 632. The Power Styramide(TM) Signal Amplification (PSA(TM)) system is a highly sensitive method for detecting low-abundant targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). By utilizing bright and photostable mFluor(TM) dyes, Styramide(TM) conjugates deliver results of unparalleled sharpness and precision, surpassing the sensitivity of standard ICC/IHC/ISH methods by over 100 times, all the while reducing the consumption of primary antibodies. Like tyramide signal amplification (TSA), PSA(TM) leverages the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The enhanced reactivity of Styramide(TM) radicals over tyramide ensures faster more robust labeling of the target, leading to fluorescence signals that are 10-50 times greater than those generated by tyramide (TSA) reagents. The mFluor(TM) Blue 630 Styramide uses the bright red fluorescent dye mFluor(TM) Blue 630 (Ex/Em = 470/632 nm) to label targets in situ. mFluor(TM) Blue 630 is well-excited by the 488 nm laser line and exhibits minimal crosstalk in complex multicolor analysis with blue and green fluorescent probes.