mFluor(TM) Blue 583 SE

Correction Factor (260 nm): 1.17. Correction Factor (280 nm): 0.35. Extinction coefficient (cm^-1 M^-1): 45000 (PBS). Excitation (nm): 498. Emission (nm): 585. AAT Bioquest's mFluor(TM) dyes are developed for multicolor flow cytometry-focused applications, in particular, for spectral fluorescence flow cytometry. mFluor(TM) Blue 583 dye is well excited with blue laser at 488 nm. It has a huge Stokes shift with emission ~583 nm. mFluor(TM) Blue 583 dyes are water-soluble, and the protein conjugates prepared with mFluor(TM) Blue 583 dyes are well excited at 488 nm to give bright red fluorescence. mFluor(TM) Blue 583 dye and conjugates are excellent blue laser-excitable reagents for flow cytometry applications. It is a unique fluorochrome for spectral flow cytometry since there are very few existing dyes that have this spectral profile. Advances in spectral flow cytometers have expanded applications and capabilities beyond conventional flow cytometry. The new color of mFluor(TM) Blue 583 provides a new option for spectral flow cytometry analysis, enabling researchers and scientists to investigate an increasing number of molecules of interest. Compared to RPE, mFluor(TM) Blue 583 dyes are much more photostable, making them readily available for fluorescence imaging applications while it is very difficult to use RPE conjugates for fluorescence imaging applications due to the rapid photobleaching of RPE conjugates.