Hoechst 33342 (hydrochloride)

Hoechst 33342 is a cell-permeable, benzimidazole dye that stains DNA by binding to the minor groove of adenine and thymine-rich sequences. It emits blue fluorescence (excitation 350 nm/emission maximum 461 nm) when bound to double stranded DNA and is useful as a marker of nuclei for cell cycle studies and to distinguish nuclear morphology in apoptotic cells.Formal Name: 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole, trihydrochloride. CAS Number: 875756-97-1. Synonyms: Bisbenzimide, HOE 33342, NSC 334072. Molecular Formula: C27H28N6O . 3HCl. Formula Weight: 561.9. Purity: >98%. Emission: 461 nm. Excitation: 350 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 20 mg/ml, PBS (pH 7.2): 5 mg/ml. lambdamax: 239, 272, 355 nm. SMILES: CN1CCN(C2=CC(NC(C3=CC=C(N=C(C4=CC=C(OCC)C=C4)N5)C5=C3)=N6)=C6C=C2)CC1.Cl.Cl.Cl. InChi Code: InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33,,,/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31),3*1H. InChi Key: JABNPSKWVNCGMX-UHFFFAOYSA-N.