DEAC-dUTP *1 mM in Tris Buffer (pH 7.5)*

Correction Factor (260 nm): 0.14. Correction Factor (280 nm): 0.12. Excitation (nm): 411. Emission (nm): 472. The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Coumarin-dUTP conjugate can be used as a blue fluorescence color with Spectrum Aqua(TM) filter set (Spectrum Aqua(TM) is the trademark of Vysis). It is chemically same to Diethylaminocoumarin-5-dUTP (Perkin Elmer's NEL455001EA).