Calcein AM

Calcein AM is a cell-permeable dye that, upon entering live cells, is cleaved by intracellular esterases, leaving membrane-impermeant calcein (Cay-16221), a fluorescent indicator with absorption and emission maxima of 494 and 517 nm, respectively. It can be used as an indicator of cell viability, cell-cell communication, cytotoxicity, or changes in intracellular calcium, fluoride, iron, or mercury. As calcein is a substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3), calcein AM is used to study these proteins and their regulation.Formal Name: N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]]-glycine, 1,1'-bis[(acetyloxy)methyl] ester. CAS Number: 148504-34-1. Synonyms: Calcein Acetoxymethyl ester, NSC 689290. Molecular Formula: C46H46N2O23. Formula Weight: 994.9. Purity: >90%. Emission: 517 nm. Excitation: 494 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMSO: 10 mg/ml, Ethanol: 10 mg/ml, Methanol: 10 mg/ml. SMILES: CC(OC1=CC2=C(C=C1CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C3(C(C=CC=C4)=C4C(O3)=O)C5=CC(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(OC(C)=O)C=C5O2)=O. InChi Code: InChI=1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15. InChi Key: BQRGNLJZBFXNCZ-UHFFFAOYSA-N.