WWL70

WWL70 is an inhibitor of alpha/beta-hydrolase domain-containing protein 6 (ABHD6, IC50 = 70 nM). It increases the expression of the adipose browning-related gene Ucp1 in differentiated 3T3-L1 mouse adipocytes and increases the oxygen consumption rate (OCR), an effect that can be blocked by the PPARalpha antagonist GW 6471 (Cay-11697), when used at a concentration of 10 µM. WWL70 (10 mg/kg per day) also increases the expression of the adipose browning-related genes Ucp1, Prdm16, Tmem26, and Tbx1 in visceral adipose tissue in mice fed a high-fat diet. WWL70 reduces adipose tissue mass and prevents glucose-intolerance and increases in body weight in mice fed a high-fat diet but does not reduce hepatic triacylglycerol levels. It increases brain levels of 2-arachidonoyl glycerol (2-AG, Cay-62160) and decreases the severity of experimental autoimmune encephalomyelitis (EAE) in wild-type, but not cannabinoid (CB) receptor 2 (CB2) knockout, mice when administered at a dose of 10 mg/kg per day starting at disease onset.Formal Name: N-methyl-N-[[3-(4-pyridinyl)phenyl]methyl]-carbamic acid, 4'-(aminocarbonyl)[1,1'-biphenyl]-4-yl ester. CAS Number: 947669-91-2. Molecular Formula: C27H23N3O3. Formula Weight: 437.5. Purity: >97%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 2 mg/ml, DMF:PBS (pH 7.2) (1:3): 0.2 mg/ml, DMSO: 1 mg/ml. lambdamax: 203, 266 nm. SMILES: O=C(Oc1ccc(cc1)c1ccc(cc1)C(=O)N)N(C)Cc1cccc(c1)c1ccncc1. InChi Code: InChI=1S/C27H23N3O3/c1-30(18-19-3-2-4-24(17-19)22-13-15-29-16-14-22)27(32)33-25-11-9-21(10-12-25)20-5-7-23(8-6-20)26(28)31/h2-17H,18H2,1H3,(H2,28,31). InChi Key: QTWNORFUQILKJL-UHFFFAOYSA-N.