WST-8

WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity. At neutral pH and in the presence of the intermediate electron acceptor, 1-methoxy phenazine methosulfate, NADPH-dependent cellular oxidoreductases, acting via plasma membrane electron transport, reduce the cell-impermeant WST-8 outside the cell to a water-soluble formazan dye with absorbance max at 460 nm. It is typically used as a cell viability indicator in cell proliferation assays.Formal Name: 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium, inner salt, monosodium salt. CAS Number: 193149-74-5. Synonyms: Water-Soluble Tetrazolium 8. Molecular Formula: C20H13N6O11S2 . Na. Formula Weight: 600.5. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 1 mg/ml, PBS (pH 7.2): 5 mg/ml. lambdamax: 255 nm. SMILES: [O-]S(C1=CC(S([O-])(=O)=O)=C(C2=NN(C3=CC=C([N+]([O-])=O)C=C3)[N+](C4=C(OC)C=C([N+]([O-])=O)C=C4)=N2)C=C1)(=O)=O.[Na+]. InChi Code: InChI=1S/C20H14N6O11S2.Na/c1-37-18-10-14(26(29)30)6-9-17(18)24-22-20(21-23(24)12-2-4-13(5-3-12)25(27)28)16-8-7-15(38(31,32)33)11-19(16)39(34,35)36,/h2-11H,1H3,(H-,31,32,33,34,35,36),/q,+1/p-1. InChi Key: VSIVTUIKYVGDCX-UHFFFAOYSA-M.