Tetramethylrhodamine methyl ester (perchlorate)

Tetramethylrhodamine methyl ester (TMRM) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry. It has two excitation peaks at 515 and 555 nm and an emission peak in the red-orange range (575 nm). Due to the polarization of the mitochondrial membrane, TMRM is taken up into healthy mitochondria. However, when the membrane is depolarized, as in apoptosis, it is not taken up or is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.Formal Name: 3,6-bis(dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-xanthylium, perchlorate. CAS Number: 115532-50-8. Synonyms: TMRM, TMR methyl ester. Molecular Formula: C25H25N2O3 . ClO4. Formula Weight: 500.9. Purity: >98%. Emission: 575 nm. Excitation: 515, 555 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: slightly soluble, PBS (pH 7.2): slightly soluble. lambdamax: 226, 255, 352, 550 nm. SMILES: O=C(OC)C(C=CC=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4.O=Cl(=O)([O-])=O. InChi Code: InChI=1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5,2-1(3,4)5/h6-15H,1-5H3,(H,2,3,4,5)/q+1,/p-1. InChi Key: PFYWPQMAWCYNGW-UHFFFAOYSA-M.