Suc-Leu-Tyr-AMC

Description: Suc-Leu-Tyr-AMC is a fluorescent substrate for calpain I and II, papain (another cysteine protease), and is used to measure the chymotrypsin-like peptidase activity of the 20S proteasome (excitation max: 360 nm, emission max: 460 nm). Suc-Leu-Tyr-AMC can also be cleaved by the Ti protease from E. coli. Smiles: CC=1C=2C(=CC(NC([C@H](CC3=CC=C(O)C=C3)NC([C@@H](NC(CCC(O)=O)=O)CC(C)C)=O)=O)=CC2)OC(=O)C1. References: Woo KM, et al. Protease Ti from Escherichia coli requires ATP hydrolysis for protein breakdown but not for hydrolysis of small peptides. J Biol Chem. 1989,264(4):2088-2091.