SL 327

The dual specific threonine/tyrosine kinase MEK is a key component of the RAS/RAF/MEK/ERK signaling pathway that regulates a diverse array of cellular processes. SL 327 is an inhibitor of MEK1 and MEK2 (IC50s = 0.18 and 0.22 µM), the kinases upstream of ERK1/2. It inhibits ERK1, MKK/p38, MKK4, JNK, and PKC at much higher concentrations (IC50s = >50, 21, >100, >100, >10 µM, respectively). Since SL 327 rapidly passes the blood-brain barrier, it has been used to dissect the effect of RAS/RAF/MEK/ERK signaling pathway inhibition on behavior, including long-term memory, spatial learning, and fear/operant conditioning.Formal Name: alpha-[amino[(4-aminophenyl)thio]methylene]-2-(trifluoromethyl)-benzeneacetonitrile. CAS Number: 305350-87-2. Molecular Formula: C16H12F3N3S. Formula Weight: 335.4. Purity: >95% (mixture of cis/trans isomers). Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 10 mg/ml. lambdamax: 278 nm. SMILES: N/C(SC1=CC=C(N)C=C1)=C(C#N)\C2=CC=CC=C2C(F)(F)F. InChi Code: InChI=1S/C16H12F3N3S/c17-16(18,19)14-4-2-1-3-12(14)13(9-20)15(22)23-11-7-5-10(21)6-8-11/h1-8H,21-22H2/b15-13+. InChi Key: JLOXTZFYJNCPIS-FYWRMAATSA-N.