Rhodamine B isothiocyanate (mixed isomers)

Rhodamine B isothiocyanate (RITC) is a cell-permeant fluorogenic dye most often used as a conjugate to antibodies and proteins for fluorescence detection in microscopy. It displays excitation/emission maxima of 570/595 nm, respectively, and can be used in conjunction with immunohistochemistry for multi-labeling studies. It has also been used as an anterograde and retrograde tracer in neurons and in nanoparticles to detect apoptosis.Formal Name: 9-[2-carboxy-4(or 5)-isothiocyanatophenyl]-3,6-bis(diethylamino)-xanthylium, monochloride. CAS Number: 944130-99-8. Synonyms: RBITC, RITC. Molecular Formula: C29H30N3O3S . Cl. Formula Weight: 536.1. Purity: Mixture of isomers. Emission: 595 nm. Excitation: 570 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 25 mg/ml, DMF:PBS (pH 7.2)(1:3): 0.1 mg/ml, DMSO: 15 mg/ml, Ethanol: 15 mg/ml. lambdamax: 220, 258, 285, 352, 554 nm. SMILES: CCN(CC)C(C=C1)=CC2=C1C(C3=C(C(O)=O)C=C(N=C=S)C=C3)=C4C(C=C(N(CC)CC)C=C4)=[O+]2.CCN(CC)C(C=C5)=CC6=C5C(C7=C(C(O)=O)C=CC(N=C=S)=C7)=C8C(C=C(N(CC)CC)C=C8)=[O+]6.[Cl-].[Cl-]. InChi Code: InChI=1S/2C29H29N3O3S.2ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)25-15-19(30-18-36)9-12-22(25)29(33)34,1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34,. InChi Key: ZCHFWDRVFPQJSN-UHFFFAOYSA-N.