Rhod-5N AM

Rhod-5N AM is a cell-permeant acetoxymethyl ester of the fluorescent calcium indicator rhod-5N (Cay-20441). As rhod-5N AM enters cells, it is hydrolyzed by intracellular esterases to produce rhod-5N. Rhod-5N displays a low affinity for calcium (Kd = 19 µM). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-5N has excitation/emission maxima of approximately 551 and 576 nm, respectively.Formal Name: N-(9-(4-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)-3-(2-(2-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)-5-nitrophenoxy)ethoxy)phenyl)-6-(dimethylamino)-3H-xanthen-3-ylidene)-N-methylmethanaminium bromide. Synonyms: Rhod-5N Acetoxymethyl ester. Molecular Formula: C51H56N5O11 . Br. Formula Weight: 1154.9. Purity: >90%. Emission: 576 nm. Excitation: 551 nm. Formulation: (Request formulation change), A solid. Solubility: DMSO: Soluble. SMILES: CN(C)C(C=C1)=CC2=C1C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC([N+]([O-])=O)=C4)=C3)=C(C=C/5)C(O2)=CC5=[N+](C)\C.[Br-]. InChi Code: InChI=1S/C51H56N5O21.BrH/c1-31(57)69-27-73-47(61)23-54(24-48(62)74-28-70-32(2)58)41-15-9-35(51-39-13-10-36(52(5)6)20-43(39)77-44-21-37(53(7)8)11-14-40(44)51)19-45(41)67-17-18-68-46-22-38(56(65)66)12-16-42(46)55(25-49(63)75-29-71-33(3)59)26-50(64)76-3. InChi Key: PMQMSJPQRCLPMW-UHFFFAOYSA-M.