Propidium Iodide

Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.Formal Name: 3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenyl-phenanthridinium, diiodide. CAS Number: 25535-16-4. Synonyms: PI. Molecular Formula: C27H34N4 . 2I. Formula Weight: 668.4. Purity: >90%. Emission: 617 nm. Excitation: 488-535 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 3.3 mg/ml, DMSO: 2.5 mg/ml, Ethanol: 0.2 mg/ml, PBS (pH 7.2): 2 mg/ml. lambdamax: 219, 298, 546 nm. SMILES: CC[N+](CC)(C)CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4.[I-].[I-]. InChi Code: InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20,,/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3,2*1H/q+1,,/p-1. InChi Key: XJMOSONTPMZWPB-UHFFFAOYSA-M.