NSC 95397

NSC 95397 is a 1,4-naphthoquinone-based irreversible inhibitor of Cdc25 dual-specificity phosphatases, with Ki values of 32, 96, and 40 nM for Cdc25A, Cdc25B, and Cdc25C, respectively. It displays >125-fold selectivity for Cdc25 over VH1-related dual-specificity phosphatase and protein tyrosine phosphatase 1b. NSC 95397 significantly inhibits the growth of human and murine carcinoma cells, blocking G2/M phase transition. In rat liver epithelial cells, NSC 95397 induces cell cycle arrest, which is associated with phosphorylation of EGFR, activation of ERK1/2, phosphorylation of connexin43, and downregulation of gap junctional intercellular communication.Formal Name: 2,3-bis[(2-hydroxyethyl)thio]-1,4-naphthalenedione. CAS Number: 93718-83-3. Synonyms: Cdc25 Inhibitor IV, PTP Inhibitor XXIX. Molecular Formula: C14H14O4S2. Formula Weight: 310.4. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS (pH 7.2)(1:5): 0.16 mg/ml, Ethanol: 0.33 mg/ml. lambdamax: 240, 274, 452 nm. SMILES: O=C1C(SCCO)=C(SCCO)C(C2=CC=CC=C21)=O. InChi Code: InChI=1S/C14H14O4S2/c15-5-7-19-13-11(17)9-3-1-2-4-10(9)12(18)14(13)20-8-6-16/h1-4,15-16H,5-8H2. InChi Key: MAASHDQFQDDECQ-UHFFFAOYSA-N.