Necrostatin-1 Inactive Control

Necrostatin-1 Inactive Control (Nec-1i) is a demethylated variant of necrostatin-1 (Nec-1, Cay-11658), a RIP1 kinase inhibitor. While both Nec-1 and Nec-1i inhibit the immune regulator indoleamine 2,3-dioxygenase, Nec1i is ~100-fold less effective than Nec-1 in inhibiting RIP1 kinase in vitro and 10-fold less potent than Nec-1 in a mouse necroptosis assay. However, equally high doses of Nec-1 and Nec-1i are reported to inhibit TNF-induced systemic inflammatory response syndrome in vivo. Nec-1i is often used as an inactive control in studies using Nec-1 to exclude nonspecific off-target effects.Formal Name: 5-(1H-indol-3-ylmethyl)-2-thioxo-4-imidazolidinone. CAS Number: 64419-92-7. Synonyms: Nec-1i, Nec-1 Inactive Control. Molecular Formula: C12H11N3OS. Formula Weight: 245.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMF:PBS (pH 7.2) (1:1): 0.5 mg/ml, DMSO: 14 mg/ml, Ethanol: 3 mg/ml. lambdamax: 220, 267 nm. SMILES: O=C(C(N1)CC2=CNC3=CC=CC=C32)NC1=S. InChi Code: InChI=1S/C12H11N3OS/c16-11-10(14-12(17)15-11)5-7-6-13-9-4-2-1-3-8(7)9/h1-4,6,10,13H,5H2,(H2,14,15,16,17). InChi Key: MPLRRPKKFHUEEL-UHFFFAOYSA-N.