mFluor(TM) Violet 530 maleimide

Absorbance (nm): 398. Extinction coefficient (cm -1 M -1): 20000. Excitation (nm): 393. Emission (nm): 543. mFluor(TM) Violet 530 dyes have fluorescence excitation and emission maxima of ~405 nm and ~530 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor(TM) Violet 530 Maleimide is reasonably stable and shows good reactivity and selectivity with thiol group. It provides a convenient tool to label the reduced form of monoclonal, polyclonal antibodies or other proteins that contains a thiol group. These conjugates are useful for flow cytometric applications with the violet laser excitation, in particular suitable for spectral flow cytometric applications. mFluor(TM) dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm). mFluor(TM) Violet dyes are optimized to be excited with a Violet laser at 405 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by Violet laser at 405 nm.