Leupeptin (hemisulfate)

Leupeptin is a reversible inhibitor of cysteine, serine, and threonine proteases that is produced naturally by Streptomyces. It has been reported to inhibit cathepsin B (Ki = 6 nM), calpain (Ki = 10 nM), trypsin (Ki = 35 nM), plasmin (Ki = 3.4 µM), and kallikrein (Ki = 19 µM), and has no effect against chymotrypsin, elastase, renin, or pepsin. Leupeptin has been shown to be protective of auditory hair cells exposed to disruptive noise or ototoxic aminoglycoside-type antibiotics. It is also widely used as a protein purification tool to prevent proteases present in tissue samples from degrading the protein of interest.Formal Name: N-acetyl-L-leucyl-N-[4-[(aminoiminomethyl)amino]-1S-formylbutyl]-L-leucinamide, hemisulfate. CAS Number: 103476-89-7. Synonyms: Acetyl-L-leucyl-L-leucyl-L-argininal. Molecular Formula: C20H38N6O4 . 1/2H2SO4. Formula Weight: 475.6. Purity: >90%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 25 mg/ml, DMSO: 16 mg/ml, Ethanol: 33 mg/ml, PBS (pH 7.2): 10 mg/ml. SMILES: O=S(O)(O)=O.O=C(N[C@H](C(N[C@H](C([H])=O)CCCNC(N)=N)=O)CC(C)C)[C@@H](NC(C)=O)CC(C)C.O=C(N[C@H](C(N[C@H](C([H])=O)CCCNC(N)=N)=O)CC(C)C)[C@@H](NC(C)=O)CC(C)C. InChi Code: InChI=1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22,1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23),(H2,1,2,3,4)/t2*15-,16-,17-,/m00./s1. InChi Key: CIPMKIHUGVGQTG-VFFZMTJFSA-N. Origin: Synthetic.